Journal: Frontiers in Behavioral Neuroscience
Article Title: Sex-Dependent Effects of Environmental Enrichment on Spatial Memory and Brain-Derived Neurotrophic Factor (BDNF) Signaling in a Developmental “Two-Hit” Mouse Model Combining BDNF Haploinsufficiency and Chronic Glucocorticoid Stimulation
doi: 10.3389/fnbeh.2018.00227
Figure Lengend Snippet: Western blotting was used to measure protein expression of TrkB receptor and phosphorylated TrkB receptor residues after exposure to chronic corticosterone (CORT), environmental enrichment (EE) or both in the dorsal hippocampus of wildtype (WT) and BDNF heterozygous (HET) mice. Results are presented as standardized protein expression relative to control. Phosphorylated TrkB residues are always divided by full length TrkB. For male results refer to (E) , which shows a significant effect of CORT ( ∧∧∧∧ ) and of EE (**) and (G) which shows a significant genotype × CORT interaction ( # ). For female results refer to (F) , which shows a significant main effect of genotype (**), significant main effect of CORT (^^), significant genotype × EE interaction ( # ) and significant EE × CORT interaction ( $ ). There were no significant changes to protein expression of FL-TrkB in males and females (A, B) , Y705 in males and female (C, D) and Y515 in females (H) . Data are mean ± SEM, n = 4–6, main effect ** P < 0.005, ∧∧∧∧ or **** P < 0.001, significant interaction # P < 0.05 and $ P < 0.005 for protein expression based on ANOVA. For full details of ANOVA results, see main text.
Article Snippet: Primary antibodies were rabbit anti-BDNF (1:200, Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA or Almone Labs, Israel), rabbit anti-NT-4 (1:200, Santa Cruz), rabbit anti-pTrkB Y705 (1:1,000, Signalway Antibody LLC, Maryland, USA), rabbit anti-pTrkB Y515 (1:1,000, Abcam, Cambridge, MA, USA), rabbit anti-pTrkB Y816 (1:500, Millipore, CA, USA), rabbit anti-TrkB (1:1,000, Santa Cruz), rabbit anti-NMDAR subunit 1 (GluNR1, 1:1,000, Cell Signaling Technology Inc, Danvers, MA, USA), rabbit NMDAR subunit 2A (GluN2A, 1:1,000, Cell Signaling Technology), rabbit NMDAR subunit 2B (GluN2B, 1:1,000, Cell Signaling Technology), or mouse anti-β-actin (1:10,000, Sigma-Aldrich).
Techniques: Western Blot, Expressing, Control
